Acoustic emission signals (AES) were used to study the relationship between activation of the Spleen Channel and changes in blood vessel volume on the auricular apex (ear tip) of eight sheep. When SP-9 was stimulated with acupressure (using an instrument to deliver 500-1000 g of pressure for 20 seconds), positive AES responses were found 87.6% of the time at SP-15 indicating transmission along the Spleen Channel and only 17.5% of the time at ST-25. The frequency and amplitude of blood vessel volume (BVV) waves recorded from the auricular apex before and after SP-9 stimulation were significantly different (p<0.05 and p0.05). When sham acupoints (non-acupoint controls) were stimulated, no significant transmission of AES occurred along the Spleen or Stomach Channels or change in the frequency and amplitude of BVV waves were found. The findings of increased blood flow to the auricular apex following SP-9 stimulation added experimental support of the traditional Chinese medicine Pi Tong Xue theory that one of the main functions of the Spleen is to govern or control blood flow. Further studies of blood flow in other parts of the body following Spleen Channel stimulation are needed.