Acupuncture has been successfully used in many species and should be a beneficial therapy in marine mammals. Many medications and treatments have been extrapolated from other species and used in dolphins. At this time, there have not been any meridians or acupuncture points plotted on the Atlantic bottlenose dolphin (Tursiops truncatus). The purpose of this study was to develop charts showing where the acupuncture points in an Atlantic Bottlenose dolphin should be located. The points were transposed from equine and canine acupuncture charts and applied to anatomy drawings, radiographs and photos of bottlenose dolphins. Also, a handheld acupoint detector was used to locate lower galvanic skin resistance on a live dolphin. These points were marked using zinc oxide ointment. Fifty-six points and descriptions of their locations were proposed.