In order to determine the optimal intensity and frequency of electrostimulation of acupoints known to enhance ruminal movement, an experimental study using Hou-san-li (ST-36), Guan-yuan-shu (equivalent to BL-21), Du-jiao and Pi-shu (equivalent to BL-18) based on an orthogonal design to evaluate a large number of variables was developed. Three experimental factors which included acupoint selection, electro-acupuncture (EA) intensity, and EA frequency were evaluated for their effect on rumen contractions associated with electrostimulation. Optimal settings determined were stimulation of Guan-yuan-shu with 12 Volt intensity and 30 Hertz frequency. Of these, the setting of EA intensity was the most important factor for effective acupoint stimulation. A number of other factors with variable positive effects included acupoint selection, differences in cattle type and the appearance of animal comfort during the procedure. Animals which developed slight, rhythmical quivering of abdominal or local muscle during the procedure generally indicated that optimal settings for electrostimulation had been found. In addition to determination of optimal settings for EA stimulation of rumen movement, it was demonstrated that EA stimulation was dependent on an intact vagus nerve. There was loss of ruminal movement in animals undergoing vagotomy and following atropine injection. The similarities of the injection of neostigmine methysulfate and electro-acupuncture on ruminal contraction were compared. In conclusion, this experimental study demonstrated that EA stimulation of select acupuncture points with appropriate intensity and frequency will exert an effect on rumen movement, which will give veterinarians a new approach to the treatment of rumen movement diseases.