Medical records of all dogs diagnosed with hyperadrenocorticism and treated with acupuncture and Chinese herbal medicine between 2004 and 2013 were reviewed. Twelve dogs met inclusion criteria and 8/12 also had pre-treatment adrenocorticotrophic hormone (ACTH) stimulation tests, urine cortisol-creatinine ratios (UCCR) and/or basal serum cortisol levels. Dogs had received acupuncture for 5-42 months at the time of review. Mai Men Dong (Ophiophogon Formula®) alone or combined with modified Jin Gui Shen Qi (Rehmannia 14®) or combined with modified Chai Hu Shu Gan (Liver Happy®), or modified Xia Xiao Fang (Rehmannia 11®) alone were administered to all dogs for 12-45 months. No adverse side effects were seen. Following treatment, polyuria, polydipsia and panting resolved in all 12 dogs. Lethargy improved more than 50% in all dogs. Polyphagia resolved in 7/12 dogs and improved over 50% in 5/12 dogs. Skin and hair changes and distended abdomens were more resistant, but improved more than 50% in 7/12 and 5/12 dogs respectively. Following a mean± standard deviation (M±SD) of 21.9±13.35 months of treatment, 8/8 dogs studied had significantly (p<0.05) reduced post-ACTH stimulation test results and 7/8 had returned to normal levels. All 5 dogs studied had significantly (p<0.05) improved UCCR following 20.8±13.27 months of treatment and 3 dogs had normal values. Basal cortisol levels returned to normal levels in 2/2 dogs studied after a M±SD 8±4.24 months of treatment. Based on the treatment results in 12 dogs, acupuncture and Chinese herbal medicine may be a safe and effective alternative to conventional medication and surgery for the treatment of some dogs with hyperadrenocorticism.