The objective of this retrospective study was to determine the efficacy of an integrative treatment regime that combines the Chinese herbal medicine, Gui Pi Tang (GPT), with an antibiotic (doxycycline) with or without vitamin K1 for treating dogs with thrombocytopenia. The study was carried out on medical records of dogs with a platelet count (PL) less than the normal range of 200×109/L. Among the 74 subjects, there were four groups: (Group A) treated with GPT +doxycycline; (Group B) treated with GPT + doxycycline + vitamin K1 injection; (Group C) treated only with doxycycline; (Group D) treated with doxycycline + vitamin K1. There were 8 study assessment time points: Week 0 (baseline) through Year 3. Statistical comparisons focused on improvements in PL (mean platelet increase, recovery rate). Except for Week 1, Group (A+B/GPT treatment) had significantly greater PL improvements (p<0.001) at all study assessment timepoints than those in (C+D/non- GPT treatment). With respect to the PL recovery rate, subjects in Group (A+B) had 74.2% of the study dogs recover to normal PL reference range by Month 1 and 89.5% by Year 3; whereas Group (C+D) had recovery rates of 21.4% and 31.3% for Month 1 and Year 3, respectively. These differences were statistically significant from Month 1 through Year 3 (p≤0.001). Gui Pi Tang demonstrated equal statistically significant efficacy in treating thrombocytopenia associated with blood borne parasitic infection or idiopathic immune-mediated disease. Results from this retrospective study suggest that integrating GPT with doxycycline was more effective for treating canine thrombocytopenia than doxycycline alone.