Zhe Ba Wei (Eight Zhe Herbs or Z-8) is a combination of eight traditional Chinese medicinal plants: Mai Men Dong (Ophiopogon), Xuan Shen (Scrophularia), Yu Jin (Curcuma), Ju Hua (Chrysanthemum), Bai Shao (Paeonia), Bai Zhu (Atractylodes), Zhe Bei Mu (Fritillaria) and Yan Hu Suo (Corydalis). In Traditional Chinese Medicine, one or more of these herbs are often part of herbal formulas used to treat infectious diseases. The objective of this study was to evaluate the effects of the oral administration of each individual herb of Zhe Ba Wei at three different doses on the humoral immune response in mice immunized with ovalbumin (OVA) antigen. One hundred and twenty five mice were randomly divided into twenty five groups of five mice each and an herbal solution composed of one of the eight Zhe Ba Wei herbs at either a low, medium or high dose (24 groups) or oral saline as a control (1 group) was orally administered for 4 days. After 4 days of oral herbs, all mice were injected subcutaneously with OVA (10 μg) in 0.89% saline solution twice, two weeks apart. Two weeks after the second OVA injection, OVA specific IgG and the IgG subclass responses were measured to evaluate the immune response in each group. Oral administration of Bai Zhu (Atractylodes) at all three doses and Mai Men Dong (Ophiopogon) and Xuan Shen (Scrophularia) at high doses significantly enhanced the serum OVA-specific IgG, IgG2a, IgG2b and IgG3 responses and therefore potentiated the humoral immune response in mice.