Acoustic emission signals (AES) were studied at 87 acupuncture points and 43 control points along 14 meridians in 223
sheep in order to find an objective method to demonstrate propagated sensation along meridians and localize acupuncture
points on the meridians of animals. The frequency and amplitude of the AES were found to be different than those of the
electromyogram, electroencephalogram and electrogastrogram. It was also found that AES were propagated along the
meridian in both directions from the site of stimulation at a speed about 6.67 cm/sec. The AES were also shown to be
associated with various types of tissues especially muscles and nerves. The AES were present during life but disappeared
on average 4 hours after death.