Equine Anhidrosis or non-sweating syndrome affects up to 20% of horses in Florida and leads to poor performance, reduced quality of life and increased morbidity and mortality from overheating. There is no specific conventional therapy which is consistently effective and moving affected horses to a cooler climate is often necessary. In this retrospective study, the medical records of 32 horses which were presented with anhidrosis to the Acupuncture Service at the University of Florida from January 2004 through January 2007 were reviewed. Eighteen of the horses met the inclusion criteria of having complete medical records, being treated with both acupuncture and Chinese herbal medicine and the clients were able to be contacted for follow-up assessment questions. Clients were asked to rank the severity of the horse’s anhidrosis before and after treatment from 0-5 based on a defined anhidrosis scale. They were also asked to report their impression of the overall success of treatment using a numerical scale from 0-3. According to the difference between the severity scores before and after treatment for anhidrosis analyzed using a General Linear Model, acupuncture and Chinese herbal medicine significantly reduced the clinical signs associated with anhidrosis (p<0.01). Further controlled prospective studies are needed to confirm these findings.