The cutaneous electrogastrogram (EGG) is an accurate, non-invasive method useful to monitor changes in the amplitude and frequency of the myoelectrical activity of the pyloric sphincter and body of the stomach. The objective of this study was to determine the effects of electro-acupuncture (EA) at ST-36 on the EGG compared to the electrical stimulation of a non-acupoint and then to evaluate the effects of three receptor antagonists, atropine, pentolamine and cimetidine, and two receptor agonists, pilocarpine and epinephrine, on the EGG during EA at ST-36. After a 12 hour fast, the mean±standard deviation of the amplitude and frequency of the EGG of 32 rabbits was compared before and during EA at 20 Hz for 15 minutes of ST-36 and a sham acupoint. The results were statistically compared with a one-way analysis of variance (ANOVA). EA of ST-36 significantly increased or decreased the amplitude of the myoelectric activity of the pylorus and body of the stomach (p<0.01) depending on whether the resting amplitude was relatively low or high. This finding supports the bidirectional regulation of the stomach by ST-36 found experientially in clinical practice. Both atropine and phentolamine significantly reduced the excitatory effect of EA at ST-36 on the EGG amplitude respectively (p <0.05) suggesting that the ST-36 regulatory pathway may involve M-cholinoceptors and α-adrenoceptors. There was no significant change in amplitude or frequency of the EGG at either recording site when a non-acupoint was stimulated.